Association of positively selected eIF3a polymorphisms with toxicity of platinum-based chemotherapy in NSCLC patients

Aim:

Eukaryotic translation initiation factor 3 subunit A (eIF3a) plays critical roles in regulating the initiation of protein translation, and eIF3a is highly expressed in lung cancer. In this study, we investigated the association of the positively selected SNPs of eIF3a with the response to and toxicity of platinum-based chemotherapy in Chinese patients with non-small cell lung cancer (NSCLC).

Methods:

SNP data for eIF3a locus were downloaded from HapMap database. For each SNP, haplotype, LD profile and population differentiation were analyzed. The long-range haplotype (LRH) test was employed to identify positively selected SNPs of eIF3a. A total of 325 NSCLC patients were enrolled and genotyped for these SNPs.

Results:

Five positively selected (rs1409314, rs4752219, rs4752220, rs7091672 and rs10510050) and 5 non-positively selected SNPs (rs10886342, rs11198804, rs2275112, rs10787899 and rs4752269) were identified in the LRH test. However, none of them was correlated with the platinum-based chemotherapy response. In contrast, 4 of the positively selected SNPs (rs1409314, rs4752219, rs4752220 and rs7091672) were significantly correlated with the toxicities tested (neutropenia, anemia, thrombocytopenia, emesis and hepatotoxicity). In addition, rs10510050 was significantly correlated with thrombocytopenia, emesis and hepatotoxicity. None of the 5 non-positively selected SNPs was correlated with the 5 toxicities.

Conclusion:

The positively selected SNPs of eIF3a are significantly correlated with platinum-based chemotherapy toxicities in Chinese NSCLC patients.     

膝关节损伤患者关节镜术后中药熏洗治疗的应用与护理

目的：探讨膝关节损伤患者关节镜术后中药熏洗治疗的应用与护理的临床效果。方法将我院骨科行膝关节镜手术的膝关节损伤患者60例,均在硬膜外麻醉下实施了关节镜手术治疗。并按照数字表法随机分为两组,对照组30例给予术后护理及功能锻炼;观察组30例在对照组的基础上实施中药熏洗,并比较两组的临床效果。结果两组患者治疗4周后,观察组的总有效率为93.33%明显高于对照组的73.33%(χ2＝4.32,P＜0.05),两组治疗后均可降低膝关节液中IL-1含量,且观察组疗效显著地优于对照组（P＜0.05),察组治疗后疼痛程度与对照组比较差异有统计学意义（P＜0.05),观察组治疗后肿胀程度与对照组比较差异有统计学意义（P＜0.05)。结论膝关节损伤关节镜术后给予患者中药熏洗治疗及功能性锻炼及护理,临床效果十分明显,可以有效的减少膝关节损伤的并发症,促进膝关节功能的快速恢复,提高患者的生活质量,值得临床推广。     

中药雾化吸入预防开胸术后肺部并发症的观察与护理

目的：观察中药雾化吸入对开胸术后肺部并发症的预防作用与护理。方法将60例开胸术患者随机分为两组,治疗组（30例）和对照组（30例）。2组均采用氧驱动雾化吸入方法。治疗组采用中药雾化,并给予心理护理及雾吸指导。对照组采用临床常规雾化药物及一般护理。结果两组患者咳痰量都出现明显下降趋势,治疗组患者的血气水平、血液炎症等指标好于对照组,并且治疗组肺炎发生率（3.33%）、肺不张发生率（6.67%）明显少于对照组肺炎（20%）、肺不张（26.77%）的发生率。经调查,治疗组的满意度（87.5%）高于对照组（70%）。结论中药雾化吸入和正确的护理可显著减少开胸术后患者肺部并发症,并有效提高护理满意率。     

中药贴敷辅助红外线治疗乳腺增生的疗效观察

目的 观察分析采用中药贴敷辅助红外线的方法对治疗乳腺增生的临床疗效,旨在为临床上治疗乳腺增生提供参考和依据.方法 选取我院2012年6月至2013年6月收治的100例乳腺增生患者,将他们随机分为两组,观察组50例给予中药贴敷辅助红外线的治疗方法,对照组50例仅给予红外线治疗的方法,观察比较两组患者的临床表现情况.结果 观察组的总有效率为96.00％,对照组的总有效率为80.00％,两组比较差异明显,具有统计学意义(P＜0.05);观察组患者均未复发,对照组的临床复发率为10.00％,两组比较差异明显,具有统计学意义(P＜0.05).结论 在治疗乳腺增生采取中药贴敷辅助红外线的方法,能明显提高改善其临床疗效,降低临床复发率,对治疗乳腺增生具有积极意义,值得临床上推广和应用.     

骨科患者万古霉素使用的专项点评

目的 通过骨科万古霉素使用情况的专项点评,促进其合理使用。方法 调取本院骨科2011—2013年使用万古霉素的病历,对其适应症、用法用量、疗程、序贯治疗、病原学检查5个方面进行合理性点评。结果 本院骨科不存在滥用万古霉素,重视病原学检查。但存在选药或疗程不合理、药液稀释浓度高以及医师对病原学检查认识不全面等现象。结论 开展万古霉素的专项点评,及时干预使用过程中存在的问题,从而促进其合理使用。     

中药泡足与穴位按摩对产后宫缩痛临床疗效的应用研究

目的 研究分析中药泡足及穴位按摩减轻经产妇产后宫缩痛的临床疗效.方法 入组2013年阴式分娩的经产妇200例,随机分成4组各50例,中药泡足组选用艾叶加宽筋滕粉热水泡足,穴位按摩组选用三阴交、足三里和血海穴位按摩,中药泡足+穴位按摩组采用艾叶加宽筋滕粉热水泡足加三阴交、足三里和血海穴位按摩,对照组给予基础治疗如改变体位、腹部热敷或按摩等,采用视觉模拟量表评分(visual analog scale,VAS)对4组产妇在产后第1～3天进行疼痛强度评估.结果 中药泡足组、穴位按摩组、中药泡足+穴位按摩组在产后第1天产后宫缩痛疼痛评分显著低于对照组,差异具有统计学意义(P＜0.05).结论 对经产妇选用中药泡足、穴位按摩或联合使用能有效减轻产后宫缩痛,增加产妇舒适度,促进产妇康复.     

中药治疗糖尿病难愈性创面愈合的研究进展

摘 要 难愈性创面是糖尿病的常见并发症,目前糖尿病难愈性创面及其治疗手段引起人们的广泛关注。本文就中药对糖尿病创面愈合的影响从促进成纤维细胞的增殖、促进肉芽组织生成、增加生长因子分泌、减少创面瘢痕及临床研究等方面进行了系统综述,以期为中药治疗难愈性创面愈合提供科学依据。     

静脉用药调配中心营养液配置不合理处方用药分析

目的：分析我院住院患者中营养液医嘱不合理用药处方,提高在营养液使用中的合理用药水平。方法对我院静配中心2014年1－12月住院长期医嘱中的营养液医嘱不合理用药部分进行分类,总结与分析。结果2014年1－12月份共配置营养液医嘱1618袋,出现营养液不合理处方67张。其中第1季度28张,占41．79％;第2季度14张,占20．90％,第3季度11张,占16．42％,第4季度14张,占20．90％。主要涉及的内容有：配伍禁忌、用药剂量过大,或不符合浓度要求、药物间相互作用等。结论通过静配中心药师对医嘱的审核和干预,能有效地降低和避免不合理用药的产生,促进临床安全、合理的用药。     

Deciphering the therapeutic mechanisms of Xiao-Ke-An in treatment of type 2 diabetes in mice by a Fangjiomics approach

Aim:

Xiao-Ke-An (XKA) is a traditional Chinese medicine (TCM) formula for the treatment of type 2 diabetes (T2D), and the effective ingredients and their targets as well as the mechanisms of XKA remain to be elucidated. In this study we investigated the therapeutic mechanisms of XKA in the treatment of T2D in mice using a Fangjiomics approach.

Methods:

KKAy mice feeding on a high-fat diet were used as models of T2D, and were orally treated with XKA (0.75 or 1.5 g·kg⁻¹·d⁻¹) for 32 d. Microarray mRNA expression data were obtained from the livers of the mice. Differentially expressed genes (DEGs) were identified by reverse rate analysis and ANOVA analysis. The compounds in XKA were identified by LC-MS analysis or collected from TCM databases. The relationships between the compounds and targets were established by combining the DEGs with information derived from mining literature or herb target databases. Relevant pathways were identified through a Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis using WebGestalt.

Results:

The compound-target-pathway network based on compounds identified by LC-MS analysis (NCA) included 20 constituent compounds, 46 targets and 36 T2D-related pathways, whereas the compound-target-pathway network based on compounds collected from databases (NCD) consisted of 40 compounds, 68 targets and 21 pathways. In the treatment of T2D, XKA might act mainly by improving carbohydrate and lipid metabolism, as well as ameliorating insulin resistance, inflammation and diabetic vascular complications.

Conclusion:

The network-based approach reveals complex therapeutic mechanisms of XKA in the treatment of T2D in mice that involve numerous compounds, targets, and signaling pathways.